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H2O2-induced cortactin tyrosine phosphorylation in syf triple-deficient MEF correlates with Fer activation. (A) Tyrosine phosphorylation of Fer and Ctn was assessed by IP/IB (Fer) or IB (Ctn) in H2O2-treated wt and syf MEF. (B) A plot of Fer pY to Fer ratios versus Ctn pY to Ctn ratios from four independent experiments reveals a strong biphasic correlation in the level of activation of these proteins in response to H2O2.
