Figure 1. Exogenous glutamine leads to an glutamine concentration in control and undercut slices.

Amino acid levels were measured in ethanol extracts from control (n=6) and undercut slices (n=6) incubated in oxygenated interface chambers for 2 hours in ACSF with or without 0.5 mM glutamine. Results demonstrate that in the absence of added glutamine, tissue levels of the amino acid are significantly lower (p<0.05, *) in undercut samples than in controls. There are also statistically significant increases (p<0.03** for control and p<0.0003*** for undercut) in glutamine levels in both control and undercut tissue in response to exogenous glutamine with a 50% increase in glutamine content in control slices and a 160% increase in undercut slices. Differences in glutamine concentrations in the control versus undercut slices treated with glutamine are statistically significant (p<0.05), but glutamate and GABA levels are not significantly altered in either undercut or control slices in response to glutamine.