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. Author manuscript; available in PMC: 2007 Aug 24.
Published in final edited form as: Neurobiol Dis. 2006 Oct 27;25(2):230–238. doi: 10.1016/j.nbd.2006.08.025

Figure 6. Glutamine induces spreading depression like events, which are attenuated by MeAIB.

Figure 6

A) Analysis of undercut slices demonstrates frequent large negative field potentials (e.g. panel D) after the addition of 250μM glutamine in 9 of 13 undercut slices from 8 animals. B) This effect is attenuated by the addition of MeAIB (10mM) with no events in any of 13 slices during 20 minute recording periods. After washout of MeAIB in the continued presence of 250 μM glutamine, 4 slices exhibit at least one large depolarization event. Each slice was recorded for a total of 45 minutes after initial exposure to glutamine. Cumulative event analysis demonstrates spreading depression like events in control and undercut cortex, with events appearing more frequently as glutamine concentration is increased. Field recordings were monitored for 10 min at each glutamine concentration. D). Sample tracing of a spreading depression event depicts typical negative potential lasting ~30 sec. Brief transient events are stimulus artifacts associated with evoked synaptic potentials.