Skip to main content
. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Virol Methods. 2007 Jan 16;141(2):146–153. doi: 10.1016/j.jviromet.2006.12.008

Fig. 3.

Fig. 3

Large scale inactivation testing. Extracts from 5 plants inoculated with 100 μg of inactivated or active wild type CPMV, respectively, were analyzed by RT-PCR for the cowpea housekeeping gene S15a (A and B), RT-PCR for the CPMV S coat protein gene (C and D), and CPMV specific Western dot blot (E and F). The RT-PCR reactions (lanes 2–6) were run on 1.2% agarose gel pre-stained with EtBr (Invitrogen, San Diego, CA). Lane 1 is the 1 Kb Plus DNA Ladder (Invitrogen, San Diego, CA). PCR fragments derived from the cowpea housekeeping gene S15a and CPMV S coat protein gene are indicated by open and closed triangles, respectively.

HHS Vulnerability Disclosure