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Formation of DNA–TrwCR covalent complexes. (A) Silver-stained SDS–PAGE gel of TrwCR(wt) incubated with different oligonucleotide substrates: R(12+4) (lane 1); R(12+18) (lane 3); a mixture of R(12+4) and R(12+18) (lane 2). The new band that appears in lane 2 corresponds to the formation of a ternary complex (with bound 4-mer and 18-mer oligonucleotide moieties (see text). (B) Same as in (A), except that reactions were carried out using TrwCR(Y26F).
