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Rga2 underwent transient Cdc28-dependent hyperphosphorylation around bud emergence during yeast growth. Elutriated G1 cells of ZR13 (WT) and ZR9 (hgc1Δ) were released into yeast growth conditions in the presence or absence of 1NM-PP1. Aliquots were harvested at the indicated times for generating budding indices (A) and for αMyc Western blotting (B).
