Table 2b.

Steroid levels by MS/MS during ACTH stimulation in subjects with AI (males and females combined)

	Steroid level 0 mins	Steroid level 30 mins	Steroid level 60 mins	Steroid Peak (maximum response to ACTH stimulation)	Significant difference in Peak2 vs 1
cortisol (μg/dL)
AI	3.06 ± 2.56	7.24 ± 5.42	8.71 ± 6.46	8.80 ± 6.36
Secondary AI	2.86 ± 2.28	8.96 ± 4.94	11.07 ± 5.7	11.07 ± 5.70	p = 0.04
Primary AI	3.41 ± 3.10	4.30 ± 4.83	4.65 ± 5.16	4.90 ± 6.01
Aldosterone (ng/dL)
AI	3.53 ± 3.01	9.37 ± 7.65	8.61 ± 7.45	9.95 ± 7.94
Secondary AI	4.53 ± 3.29	13.19 ± 7.20	11.75 ± 7.69	13.70 ± 7.72	p = 0.004
Primary AI	1.82 ± 1.12	2.84 ± 1.73	3.24 ± 1.45	3.54 ± 1.87
11-Deoxycortisol (ng/dL)
AI	45 ± 52	188 ± 117	208 ± 136	222 ± 132
Secondary AI	29 ± 31	220 ± 94	265 ± 112	270 ± 110	p = 0.039
Primary AI	71 ± 65	134 ± 132	111 ± 110	140 ± 123
DHEA (ng/dL)
AI	49 ± 67	59 ± 83	70 ± 105	92 ± 110
Secondary AI	55 ± 74	76 ± 95	90 ± 122	111 ± 126	p = 0.35
Primary AI	37 ± 53	29 ± 47	38 ± 47	60 ± 59
17-OH progesterone (ng/dL)
AI	36 ± 41	85 ± 56	91 ± 65	124 ± 120
Secondary AI	32 ± 31	93 ± 53	105 ± 69	149 ± 138	p = 0.25
Primary AI	42 ± 52	71 ± 62	66 ± 50	81 ± 52
Androstenedione (ng/mL)
AI	0.30 ± 0.24	0.38 ± 0.33	0.32 ± 0.32	0.49 ± 0.37
Secondary AI	0.32 ± 0.27	0.46 ± 0.38	0.38 ± 0.37	0.49 ± 0.37	p = 0.26
Primary AI	0.26 ± 0.21	0.25 ± 0.16	0.21 ± 0.14	0.31 ± 0.15
DHEA-S (μg/dL)
AI	17 ± 25	20 ± 26	21 ± 20	25 ± 27
Secondary AI	15 ± 16	19 ± 19	22 ± 20	25 ± 21	p = 0.99
Primary AI	21 ± 30	22 ± 32	18 ± 20	25 ± 33