Skip to main content
. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Dev Biol. 2007 Apr 27;307(1):114–126. doi: 10.1016/j.ydbio.2007.04.027

Fig. 1. Targeting strategy for generating the conditional allele of Hand2.

Fig. 1

A–C: Schematic representation of the strategy used to insert loxP sites and the neomycin cassette flanked by FRT sites into the Hand2 locus. D: Schematic representation of targeted allele after neomycin cassette has been recombined out with FLT recombinase. E: Schematic representation of targeted allele after recombination with Cre. F, G: PCR analysis of the 3′ and 5′ ends of the targeted Hand2 gene. H: PCR analysis of the conditional Hand2 allele showing that the neomycin cassette was removed by FLT recombinase. A details description of the targeting strategy is presented in Materials and Methods