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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Dev Biol. 2007 Apr 27;307(1):114–126. doi: 10.1016/j.ydbio.2007.04.027

Fig. 4. Hand2 is not required for colonization of sites of sympathetic ganglion formation.

Fig. 4

The level of Hand2 transcript was examined in wild-type (A) and Hand2loxP/−; Wnt1-Cre/+ (B) embryos by in situ hybridization. Hand2 expression (arrowheads) is undetectable in mutant embryos. C–D: Precursors of the SNS were identified by in situ hybridization with c-ret No difference in c-ret expression was detected between wild-type (C) and Hand2loxP/−; Wnt1-Cre/+ (D) embryos. E-F: The size of sympathetic paravertebral ganglia was analyzed by marking all sympathetic neurons genetically by Wnt1-Cre and R26R genes. R26R line was crossed into Wnt1-Cre/+ (E) and Hand2loxP/−; Wnt1-Cre/+ (F) lines, resulting in β-galactosidase activation in all NC cells and their descendents. The number of sympathetic neurons was not affected by the loss of Hand2. Arrowheads; sympathetic trunk. DRG: dorsal root ganglia.