Figure 2.
Native 12.5% acrylamide gel electrophoresis of PAB0955. The concentration of PAB0955 sample was 4 mg ml−1 in a buffer containing 20 mM NaH2PO4 pH 7.2 and 50 mM NaCl. The migration was performed at 200 V. The migration of the protein without DTT and after incubation in 10 mM DTT are shown in the left and right column, respectively. The dimeric state clearly prevails in the presence of DTT.