Figure 2.

Self-rotation function of the Moraxella sp. TAE123 psychrophilic ADH, κ = 180° section. Radial coordinate, ω; angular coordinate, ϕ. The function was calculated with reflections in the resolution range 14–3 Å and an integration radius of 18 Å using the program POLARRFN from the CCP4 program package (Collaborative Computational Project, Number 4, 1994 ▶). Contouring of the map starts at the 2σ level with steps of 0.5σ. Two molecular dyads of the ADH tetramer are visible at polar angles (ω, ϕ) of (77, 90°) and (13, 270°), while the third molecular dyad is parallel to the crystallographic x axis.