SDS–PAGE and Western blot analysis of the dissolved crystals. (a) SDS–PAGE of the dissolved crystals (5 µg each). Lane M, low-molecular-weight markers; lane 1, dissolved crystals of CARDO-OJ3; lane 2, dissolved type I crystals of the complex; lane 3, dissolved type II crystals of the complex; lane 4, dissolved crystals of CARDO-FCA10. (b) Western blots using anti-CarAcCA10 antibody. Lane numbers are the same as in (a).