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Purification of Mtb-AK protein expressed in E. coli and protein electrophoresis under denatured (a) and native conditions (b) in 8–16% polyacrylamide gradient gels. (a) SDS–PAGE. Lane 1, molecular-weight (MW) markers; lane 2, crude cell extract; lanes 3–14, elution fractions from Ni2+-affinity column. (b) Native PAGE. Lane 1, MW markers; lane 2, crude cell extract; lanes 3–10, elution fractions from the same column.
