Table 1. Statistical analysis of X-ray diffraction data for native and SeMet complex crystals.
Native type II complex | SeMet type II complex | |
---|---|---|
X-ray source | CHESS (F2 station) | NSLS (X8C beamline) |
Wavelength (Å) | 0.9790 | 0.9788 |
Detector | CCD | CCD |
Crystal parameters | ||
Space group | P212121 | P212121 |
Unit-cell parameters | ||
a (Å) | 45.57 | 45.21 |
b (Å) | 52.22 | 52.34 |
c (Å) | 156.26 | 154.69 |
Resolution (Å) | 2.1 (2.18–2.10) | 2.0 (2.07–2.00) |
Mosaicity (°) | 0.53 | 0.65 |
Solvent content (%) | 48 | 48 |
Molecules per AU (heterodimers) | 1 | 1 |
Data processing | ||
No. observed reflections | 88650 | 300975 |
No. unique reflections | 22173 | 25648 |
Completeness (%) | 99.0 (95.8) | 99.6 (96.3) |
Rsym (%) | 5.9 (18.1) | 10.3 (35.4) |
Average I/σ(I) | 15.8 (4.8) | 25.81 (3.0) |