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RT-F155V-H can incorporate rUTP or rGTP into products, using poly(rA)/oligo(dT) or poly(dC)/oligo(dG) as template/primer. Reactions were performed with the indicated template/primer, enzymes, and labeled substrates, and elongated products were assayed by spotting on DE81 paper, washing, and autoradiography. Mutant RT-F155V-H was uniquely able to incorporate ribonucleotides.
