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Effects of Ap5A on E. coli selenophosphate synthetase activity estimated by AMP formation. Reaction mixtures as shown in the legend to Fig. 4 were incubated with 2 μM (7 μg) E. coli selenophosphate synthetase with or without 1.5 mM NaSeH and with or without 0.1 mM Ap5A and analyzed as described in Materials and Methods.
