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. 2007 Sep 4;117(9):2723–2734. doi: 10.1172/JCI31604

Figure 1. Inhibition of RR causes mtDNA depletion during proliferation.

Figure 1

(A) Relative mtDNA copy number for wild-type primary fibroblasts treated with Triapine during high proliferation (0–24 hours) or reduced proliferation (24–48 hours) conditions. The ratio of the amount of mtDNA to nuclear DNA is plotted with the ratio in control cells arbitrarily set to 1.0. The mean ± SEM is plotted with significant statistical differences via Student’s t test indicated. (B) Relative mtDNA copy number of gemcitabine-treated wild-type primary fibroblasts. Cultures were maintained in the concentrations indicated for 48 hours with untreated (0 μM) arbitrarily set to 1.0. The mean ± SEM is shown. (C) Relative mtDNA copy number (plotted as in A) of HeLa cells with R2 or R1 expression knocked down compared with vector or short hairpin RNA control cells, respectively. R2 was reduced 50% and R1 was undetectable via Western blot. The mean ± SEM is plotted with significant statistical differences via Student’s t test indicated.