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. 2007 Sep 4;117(9):2723–2734. doi: 10.1172/JCI31604

Figure 5. ATM/RR-dependent alterations in mtDNA copy number occur independently of h-mtTFA.

Figure 5

(A, C, and D) Western blot analyses of h-mtTFA with the relative mtDNA copy number summarized in numerical form at the top of each panel based on the data presented in Figures 1, 2, and 3. Quantification of h-mtTFA levels relative to actin are plotted in A and D as described in Figure 2D. (A) Wild-type and A-T primary fibroblasts at 24 or 48 hours and wild-type primary fibroblasts untreated (C) or treated with 10 μM KU-55933 for 24 hours after a 48-hour preincubation. (B) Relative mtDNA copy number (as plotted in Figure 1) of ethidium bromide–treated (EtBr-treated) wild-type primary fibroblasts with concurrent Western blot analysis of h-mtTFA. Hours in EtBr are indicated, and numbers below actin and h-mtTFA panels indicate protein normalized to actin. (C) HeLa cells with expression of R2 stably knocked down 50% by RNAi (R2) or with empty vector (V). (D) Wild-type and A-T primary fibroblasts nonirradiated (C) or 48 hours after irradiation (IR).