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. Author manuscript; available in PMC: 2008 Jun 2.
Published in final edited form as: Brain Res. 2007 Mar 12;1151:1–11. doi: 10.1016/j.brainres.2007.03.004

Figure 5. Correlation between the number of CHT-positive cells in the hilus and the level of CHT mRNA in the hippocampus at P18.

Figure 5

(A) CHT-positive neuronal perikarya in the CA4 regions of the hippocampal formation of P18 animals were quantitated and analyzed using a semi-automated computerized morphometry system. The number of CHT-positive cells was significantly different among the three groups of animals as determined by ANOVA (p<0.005). The ANOVA was followed up with Tukey tests at a 5% procedure wise error rate. Control animals had a significantly higher number of CHT-positive cells compared to prenatally choline-supplemented animals. (B) Hippocampal RNA from P18 rats was used for RT-PCR of CHT and β-actin. CHT levels were normalized using β-actin levels and are presented as means ± SEM (n=6 per group). The levels of CHT were significantly different among the three groups of animals as determined by ANOVA (p<0.05). The ANOVA was followed up with Tukey tests at a 5% procedure wise error rate. Prenatally choline-deficient and control animals had a significantly higher amount of CHT mRNA compared to prenatally choline-supplemented animals. (C) A significant correlation between the number of CHT-positive cells in the hilus and the level of CHT mRNA in the hippocampus was observed (R=0.567 and p<0.05). S, prenatally choline-supplemented; C, control; D, prenatally choline-deficient.