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Western blot analysis of NNMT. Tumor (T) and matched adjacent healthy (N) tissue lysates were prepared as described in Materials and Methods; 50 μg of protein from 15 percent SDS-PAGE were transferred to polyvinylidene fluoride membranes. Blot was probed with anti-NNMT antibody, and detected with enhanced chemiluminescence. (cases 1–11, favorable OSCCs; cases18 and 21, unfavorable OSCCs).
