Figure 5.
Ad-GFAP-HO-1 protects wild-type astrocytes from hemin. Phase-contrast photomicrographs of astrocyte cultures, 24 hours after: A) medium exchange only; B) treatment with 10 μM hemin; the culture monolayer is no longer confluent, and most remaining astrocytes have developed a granular appearance with pyknotic nuclei; C) treatment with 10 μM hemin after 24 hour incubation with 100 MOI Ad-Null; D) treatment with 10 μM hemin after 24 hour incubation with 100 MOI Ad-GFAP-HO-1; culture monolayer remains intact. Scale bar = 50 μm.