Table 4.
Respiratory parameters of liver isolated mitochondria from pair-fed (PF), control (CON) and dexamethasone (DEX)-treated rats. (Values expressed as mean ± standard deviation for eight rats per group)
| Respiratory substrates | Parameters | DEX-treated | PF | CON | ANOVA |
|---|---|---|---|---|---|
| Glutamate | State 3 | 35 ± 16 | 26 ± 13 | 43 ± 19 | NS |
| State 4 | 5 ± 1 | 5 ± 1 | 6 ± 1 | NS | |
| RCR | 7 ± 3 | 5 ± 3 | 7 ± 3 | NS | |
| Uncoupled state | 29 ± 17 | 24 ± 13 | 46 ± 24 | NS | |
| Succinate | State 3 | 76 ± 29 | 79 ± 28 | 98 ± 16 | NS |
| State 4 | 18 ± 5 | 14 ± 3 | 18 ± 2 | NS | |
| RCR | 4.2 ± 1.4 | 5.6 ± 1.4 | 5.4 ± 0.8 | NS | |
| Uncoupled state | 93 ± 26 | 103 ± 31 | 121 ± 21 | NS | |
| TMPD/ascorbate | State 3 | 131 ± 34 | 122 ± 13 | 147 ± 27 | NS |
| State 4 | 98 ± 26 | 81 ± 16 | 96 ± 16 | NS | |
| RCR | 1.3 ± 0.1 | 1.5 ± 0.2 | 1.5 ± 0.1 | NS | |
| Uncoupled state | 167 ± 39 | 163 ± 30 | 190 ± 41 | NS |
NS: not significant.
State 3, state 4 and uncoupled state respiration expressed as natomes of oxygen/min per mg mitochondrial protein. For details of procedures see materials and methods section.