Table 5.
Respiratory parameters of gastrocnemius isolated mitochondria from pair-fed (PF), control (CON) and dexamethasone (DEX)-treated rats. (Values expressed as mean ± standard deviation for eight rats per group)
| Respiratory substrates | Parameters | DEX-treated | PF | CON | ANOVA |
|---|---|---|---|---|---|
| Pyruvate + malate | State 3 | 70 ± 39 | 105 ± 48 | 85 ± 30 | NS |
| State 4 | 8 ± 4 | 9 ± 2 | 7 ± 2 | NS | |
| RCR | 9 ± | 11.7 ± | 12.8 ± | NS | |
| Uncoupled state | 123 ± 74 | 174 ± 37 | 133 ± 41 | NS | |
| Succinate | State 3 | 88 ± 29 | 118 ± 26 | 120 ± 44 | NS |
| State 4 | 24 ± 13 | 35 ± 21 | 27 ± 16 | NS | |
| RCR | 3.7 ± | 3.4 ± | 4.4 ± | NS | |
| Uncoupled state | 123 ± 46 | 156 ± 53 | 128 ± 59 | NS | |
| TMPD/ascorbate | State 3 | 260 ± 117 | 289 ± 75 | 231 ± 61 | NS |
| State 4 | 172 ± 84 | 186 ± 58 | 156 ± 39 | NS | |
| RCR | 1.5 ± 0.2 | 1.5 ± 0.2 | 1.5 ± 0.3 | NS | |
| Uncoupled state | 385 ± 128 | 354 ± 108 | 403 ± 121 | NS |
NS: not significant.
State 3, state 4 and uncoupled state respiration expressed as natomes of oxygen/min per mg mitochondrial protein. For details of procedures see materials and methods section.