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Inhibition of Gqα binding to RGS4 by phospholipase Cβ1. Gqα-GDP (50 nM; row 1) or Gqα-GDP-AlF4− (50 nM; rows 2 and 3) was incubated with hexa-histidine-tagged RGS4 (500 nM) in the absence (rows 1 and 2) or presence (row 3) of phospholipase Cβ1 (200 nM). Ni-NTA resin was added and processed as described under Materials and Methods, and fractions were analyzed by immunoblotting (L, load; F, flowthrough; W, wash; E, eluate).
