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Effects of ANP on cGMP accumulation in cells or on guanylyl cyclase activity in broken-cell preparations. (A) ANP (1 μM) or no ANP was added to COS-7 cells transfected with pCMV5, GC-A, or GC-A E974A. (B) Membranes prepared from transfected COS-7 cells were assayed for guanylyl cyclase activity in the presence of Mg2+ and in the presence or absence of ANP/ATP.
