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DNase I protection of DNA bound to HsRad51 or RecA
| Cofactor | % DNA protected from DNase I digestion
|
|
|---|---|---|
| RecA | HsRad51 | |
| ATP | 90 | 90 |
| ATP[γS] | 95 | 79 |
| None | 91 | 30 |
The reaction was done as described in the text. The amount of DNase I used (0.067 unit/μl) was sufficient to digest all unprotected DNA molecules in 2 min. Protected DNA was the fraction that remained trichloroacetic acid-insoluble.
