Figure 3.

Surface plasmon resonance analysis of Klenow–TBD polymerase/thioredoxin interaction. (A) The binding of thioredoxin to a sensor chip that has antithioredoxin mAbs covalently attached to its surface. The open arrow indicates the start of the injection of thioredoxin, and the filled arrow indicates the start of the buffer wash. The number of RU that remain after the initial sharp decay at the start of the buffer wash are indicated by the discontinuous arrows. (B) The binding of Klenow DNA polymerase to a chip containing thioredoxin bound to the mAbs. (C) The binding of Klenow–TBD DNA polymerase to a chip containing thioredoxin bound to the mAbs.