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Effect of thioredoxin on DNA polymerase activities
| Polymerase | Specific activity, units/mg
|
|
|---|---|---|
| − thioredoxin | + thioredoxin | |
| Klenow DNA polymerase | 2100 | 2,200 |
| T7 DNA polymerase | <4 | 10,000 |
| Klenow–TBD1 DNA polymerase | 400 | 3,400 |
Polymerase activities were measured on primed single-stranded M13 mGP1-2 DNA using a 10-fold molar excess of template over enzyme as described.
