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Kinetics of oocyte maturation in response to cyclin B1 fusion proteins containing a wild-type NLS or NLSmut derived from nucleoplasmin. In vitro-synthesized RNA of each construct was microinjected into a minimum of 20 stage VI oocytes. Oocytes were monitored every 30 min to determine whether they had reached GVBD. (A) NLS–B1Ala, NLSmut–B1Ala, and cyclin B1Ala. (B) NLS–B1, NLS–B1Glu, NLSmut–B1, and NLSmut–B1Glu.
