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Soft agar transformation assay of transfected MC. Control MC, transfected with vector alone, did not grow in suspension (A), whereas cells expressing the 16-kDa N-terminal IL-1α propiece form multicellular colonies with high efficiency (B and C). Cells expressing the NLS-mutated N-terminal IL-1α propiece did not grow in suspension (D). (A–D; ×75.)
