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Nuclear targeting of N-terminal IL-1α propiece/Drosophila ADH fusion protein. Cells transfected with ADH cDNA alone demonstrated exclusively cytosolic staining (A), whereas both components of the IL-1α propiece/ADH fusion protein were concentrated in the nucleus as demonstrated by ADH staining (B) and immunofluorescence staining for the propiece (C). Cells expressing IL-1α propiece/ADH fusion protein developed large colonies in agar suspension (D). (A–C, ×750; D, ×85.)
