Figure 3.

B-MYB protein is modified following transfection with cyclin A and cyclin E. (A) SAOS-2 cells were transiently transfected with 5 μg of CMV-B-MYB and 5 μg of CMV-cyclin A or CMV empty vector. After 36 h the cells were lysed and immunoprecipitates were loaded onto a 10% acrylamide minigel (Bio-Rad) and electrophoresed. (B) SAOS-2 cells were transiently transfected with B-MYB in combination with various cyclins, and after 36 h cells were directly lysed in SDS sample buffer, boiled and loaded onto a low-percentage (7%) acrylamide/SDS gel. Western blotting was carried out as described in the text.