Figure 1 The IL31 receptor complex and IL31 are expressed in IEC. (A) mRNA expression of IL31Rα, OSMR, and IL31 as analysed by RT‐PCR analysis of mRNA derived from IEC lines as indicated. (B) Western blot analysis of total cell protein isolated from HT‐29, SW480 and HCT116 cells demonstrates expression of the long, active isoform of IL31Rα. An amount of 80 µg protein was loaded per lane. (C) Immunofluorescence staining of HCT116 cells with an IL31Rα specific antibody demonstrates cell surface expression of IL31Rα (upper panels) while no specific staining was obtained with the secondary antibody only (lower panels).