Figure 4 IL31Rα, OSMR, and IL31 mRNA expression are upregulated by proinflammatory stimuli. (A) Regulation of IL31Rα, (B) OSMR, and (C) IL31 by LPS (1 µg/ml) and the proinflammatory cytokines TNF‐α (50 ng/ml), IL1β (10 ng/ml), and IFN‐γ (1000 U/ml). HCT116 cells were stimulated for time intervals as indicated and mRNA expression was measured by quantitative PCR and normalised to β‐actin expression.