Figure 1 Bone marrow‐derived dendritic cells (DCs) effectively take up tumour antigen and secrete high levels of interleukin (IL)12 p70 after stimulation with lipopolysaccharide (LPS) and interferon (IFN)γ. DCs were derived from bone marrow of C57BL/6 mice by culture with granulocyte–macrophage colony‐stimulating factor and IL4. On day 6 of culture, DCs were incubated with UV‐irradiated, carboxyfluorescein diacetate‐succinimidyl ester (CFSE)‐labelled Panc02 cells at a ratio of 10:1. Uptake of tumour cells by CD11c⁺ DCs was determined by fluorescence‐activated cell sorting (FACS) analysis (A; the results of one representative FACS analysis out of five performed are shown). At day 6 of culture, DCs were stimulated for another 24 h with different stimuli. Culture supernatants were harvested and analysed for IL12 production by ELISA (B; data are the mean (SEM) of two independent experiments).