Figure 5 Biodistribution of transgene transcription after intra‐articular injection. To assess the presence of the transgene, heart, lung, kidney, spleen, draining lymph nodes, liver, and testis were excised and homogenised 14 days after intra‐articular injection. RNA was isolated and cDNA was synthesised (n = 5/group). Real‐time PCR was performed using transgene specific primers. The resulting threshold cycle (Ct) values were normalised to glyceraldehyde phosphodehydrogenase (GAPDH) levels and expressed as fold increase compared with control (mean (SEM)).