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. Author manuscript; available in PMC: 2007 Aug 28.
Published in final edited form as: Clin Chim Acta. 2007 Feb 11;380(1-2):145–150. doi: 10.1016/j.cca.2007.01.031

Fig. 2.

Fig. 2

Effects of sodium selenite or MSeA on Mib or IL-6-stimulated AR transactivation activity in LNCaP cells. (A) After co-transfected with PSA-6kb promoter-luciferase reporter (1 μg/well) and CMV-β-gal vectors (0.2 μg/well) for 24 h, LNCaP cells were treated with Mib or without and plus or minus MSeA or selenite as indicated. Twenty-four hours later, cell lysate was collected and transcriptional activity of PSA-6kb and expression of β-gal were determined by luciferase and β-gal assay, respectively. The relative luciferase activities were normalized by β-gals. (B) Effects of sodium selenite and MSeA on IL-6 stimulated AR transactivation activity in LNCaP cells were determined by luciferase and β-gal assays.