Figure 2.

Flow cytometry analysis with cell surface markers. Cells obtained from livers of c/ebpα^+/− (A) or c/ebpα^−/− (B) newborns and from wild-type (I) or c/ebpα^−/− (J) day 14 embryos were stained with antibodies to the mature monocyte/granulocyte surface marker Mac-1 (37) and to the mature granulocyte surface marker Gr-1 (38). Cells obtained from spleens of c/ebpα^+/− (C) or c/ebpα^−/− (D) newborns were stained with antibodies to the B lymphocyte marker B220. Cells obtained from thymus of c/ebpα^+/− (E) or c/ebpα^−/− (F) newborns were stained with T lymphocyte surface markers CD4 and CD8. Cells obtained from livers of c/ebpα^+/+ (G) or c/ebpα^−/− (H) day 16 embryos were stained with antibodies to the erythroid surface marker TER-119.