Figure 6.

TAp73 induces apoptosis of medulloblastoma cell lines. Histograms summarizing flow cytometric analysis of cell cycle distributions of: (A) transfected Daoy cell line, illustrating the sub-G0/G1 peak representing apoptotic nuclei; and (B) transfected D283 cell line in sub-G0/G1, with plasmids and treatment with VP-16 (1.5 µM), as indicated. Y-axis, % of cells with apoptotic features by flow cytometry; X-axis, transfected plasmid(s) and culture conditions. Western immunoblots also reveal that (C) Daoy cells and (D) D283 cells transiently transfected with isoform-specific siRNA reveal knockdown of their respective protein levels. D283 knockdowns reduced protein expression of the p53/p73 target, p21^Waf1, and cleavage of PARP (arrows).