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. Author manuscript; available in PMC: 2008 Mar 1.
Published in final edited form as: Development. 2007 Jan 31;134(5):999–1009. doi: 10.1242/dev.02785

Fig. 6. Vari interacts with Nrx through the PDZ domain.

Fig. 6

(A) A purified C-terminal fragment of Nrx is co-precipitated by purified Glutathione S-transferase fused to the short isoform of Vari and the Vari PDZ domain, but not GST or GST fused to the Vari-SH3 domain. (B) GST-Vari and GST-VPDZ co-precipitate Nrx but not Crb from 12–18-hour-old embryo lysates, despite the similarity of the Crb and Nrx C-terminal PDZ-binding motifs (ERLI vs EIFI). NaCl concentrations: 150, 350 and 550 mmol/l. GST, Glutathione S-transferase; IB, immunoblot antisera; VPDZ, Vari PDZ domain.