Figure 7.

Role of NADPH oxidase in gp120-induced apoptosis and cell death in human primary neurons. Cells were incubated with 1 μm of either ASO or ScO against p22phox. Control cells received only vehicle (PBS). After 40 hr, cells were stimulated with 200 pm gp120. After 6 hr of stimulation, TUNEL-positive (+ve) cells were counted manually in four different images of each of three coverslips by three blinded individuals (A). After 18 hr of stimulation, cells were subjected to MTT reduction activity assay (B), and culture medium was collected for LDH release assay (C). Values obtained from the control group served as 100%, and data obtained in other groups were calculated as percentage of control accordingly. Results are mean ± SD of three different experiments. ^ap < 0.001 versus gp120.