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. Author manuscript; available in PMC: 2007 Aug 28.
Published in final edited form as: J Biol Chem. 2002 Aug 9;277(42):39327–39333. doi: 10.1074/jbc.M111841200

Fig. 4. MBP-primed T lymphocytes induce the expression of iNOS in mouse BV-2 microglial cells.

Fig. 4

BV-2 cells were stimulated with different concentrations of MBP-primed T cells under serum-free conditions. In A, cell homogenates were electrophoresed, transferred on nitrocellulose membrane, and immunoblotted with antibodies against mouse macrophage iNOS as described under “Materials and Methods.” In B, after 6 h of incubation, Northern blot analysis for iNOS mRNA was carried out as described under “Materials and Methods.” GAPDH, glyceraldehyde 3-phosphate dehydrogenase.