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. Author manuscript; available in PMC: 2007 Aug 28.
Published in final edited form as: J Biol Chem. 2002 Aug 9;277(42):39327–39333. doi: 10.1074/jbc.M111841200

Fig. 6. Functional blocking antibodies against the α4 chain of VLA4 inhibit the ability of MBP-primed T cells to induce the production of NO in BV-2 microglial cells.

Fig. 6

MBP-primed T cells were mixed with either different concentrations of antibodies against the α4 chain of VLA4 or control IgG and rocked gently for 1 h at room temperature. Cells were centrifuged, washed twice, and added to BV-2 microglial cells at a ratio of 0.7:1 T cell:microglia. After 24 h of stimulation, supernatants were used for nitrite assay. Data are mean ± S.D. of three different experiments.