Table 2.
Disulfide bonded P. aerophilum proteins and complexes identified from 2D gels.
| Gene | Protein | Spot No. | Cysteines | Likely Disulfides# |
|---|---|---|---|---|
| PAE2842 | Hypothetical protein | 1 | 3 (57, 131, 144) | Potential heterocomplex |
| PAE3103 | NusG, transcription antitermination | 2 | 1 (6) | Probable heterocomplex |
| PAE3173 | Conserved hypothetical | 3 | 1 (130) | |
| PAE3144 | Conserved hypothetical | 4 | 1 (116) | |
| PAE2072 | Conserved protein | 5 | 1 (120) | Potential heterocomplex |
| PAE2576 | Hypothetical protein | 6 | 5 (29, 33, 67, 143, 177) | Potential heterocomplex |
| PAE2254 | Hypothetical protein | 7 | 2 (145, 154) | |
| PAE3406 | Conserved hypothetical | 8 | 3 (40, 133, 147) | |
| PAE0797 | Short chain dehydrogenase | 9 | 1 (259) | Homodimer |
| PAE2075 | Nitrilase, conjectural | 10 | 2 (141, 257) | Homodimer (257–257′) |
| PAE1689 | Citrate synthase | 11 | 2 (19, 394) | Homodimer (19, 394) |
| PAE2310 | Proline dehydrogenase | 12 | 1 (408) | |
| PAE0210 | Conserved hypothetical | 13 | 6 (75, 100, 107, 201, 265, 292) | Intramolecular |
| PAE2489 | Acetyl-CoA acyltransferase-associated | 14 | 6 (58, 61, 72, 75, 137, 173) | Intramolecular |
| PAE2701 | Conserved hypothetical | 15 | 2 (89, 114) | Intramolecular |
| PAE3161 | Hypothetical protein | 16 | 4 (23, 25, 106, 138) | Intramolecular |
See Figure 1A for corresponding spot numbers. Cysteine residue numbers are given in parenthesis.
#
Complexes were assigned as likely homomeric vs. heteromeric based on spot positions in the gel and the presence or absence of likely partners related vertically. Where possible, assignment of likely disulfide connectivity was based on analysis of conservation in multiple sequence alignments, with conserved cysteines presumed to be functionally active rather than disulfide bonded.