Figure 1.

Binding of p54(Hrr25) to Swi6 protein affinity columns. (A) Yeast extracts from BJ2168 were loaded onto either a control column (no coupled protein, lane 1) or a Swi6-coupled column (lane 2) and eluted with 1 M NaCl. (B) Extracts made from either a wild-type (lanes 1 and 2) or an hrr25Δ strain (lanes 3 and 4) were loaded onto either a control column (lanes 1 and 3) or Swi6-coupled columns (lanes 2 and 4) and eluted with 1 M NaCl. Protein molecular weight markers are indicated to the left of the gel photographs and the p54 (Hrr25) is indicated by the arrow on the right.