Figure 6.

Subcellular Localization of Yap1 during the Life Cycle of U. maydis.

(A) Compatible strains FB1yap1:3XeGFP and FB2yap1:3XeGFP were inoculated in maize seedlings. Hyphae and appressoria on the leaf surface were visualized by calcofluor staining 16 h after infection.

(B) GFP fluorescence of the same leaf area shown in (A). Hyphae that have not yet penetrated do not show fluorescent nuclei (left panel). Hyphae that have penetrated show fluorescent nuclei (arrowheads), and these could be visualized in a different focus plane, indicating that the appressoria shown in (A) had already penetrated.

(C) Fluorescent nuclei (arrowheads) in intracellularly growing hyphae 2 d after infection.

(D) Intracellular hyphae with diffuse fluorescence 6 d after infection.

Bars = 10 μm.