Figure 3.

Expression Pattern of the Ethylene Reporter EBS:GUS Is Altered in the aux1 Mutant Plants.

Activity of the EBS:GUS ethylene reporter in roots of 3-d-old etiolated seedlings grown in media not supplemented (air) or supplemented (ACC) with 10 μM ACC. Transgenic lines carrying the EBS:GUS construct were originally generated in Col-0 plants and then introgressed into aux1-7 by crossing. Two representative lines in both Col and aux1-7 backgrounds are shown. GUS staining was performed overnight. Imaging conditions were identical for all of the plants in the experiment. The images displayed are representative of at least three independent experiments with >20 seedlings examined per experiment.