Figure 5.

TOZ Expression Patterns.

In toz/+ heterozygous plants, GUS is expressed weakly at the four-cell proembryo stage (A) but strongly at the mid-globular stage (B). In the seedling, GUS staining is predominant in the shoot apex, young leaves (C), and root tips (D). In the inflorescence, the precipitate is strongest in the young stamens and carpels. Using a different staining solution that restricts the diffusion of intermediates, GUS can be localized to the nucleus in the roots (G) and also in each of the cells of the embryo sac in the mature ovule (H).