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. 2007 Jun;19(6):2053–2063. doi: 10.1105/tpc.106.047449

Figure 1.

Figure 1.

Expression of 2b Inhibits the Accumulation of All Three Classes of viRNAs.

(A) Detection of (−)-strand CMV siRNAs in the inoculated leaves of wild-type Columbia (Col-0), dcl2-1, dcl3-1, dcl4-2, dcl2-1 dcl3-1, dcl2-1 dcl4-2, dcl3-1 dcl4-2, and dcl2-1 dcl3-1 dcl4-2 plants at 5 d after infection with either CMV (wt) or CMV-Δ2b (Δ). The membrane was also probed for miR173 and U6 RNAs. Positions of the 21-, 22-, and 24-nucleotide (nt) siRNAs are indicated.

(B) Detection of CMV genomic and subgenomic RNAs (indicated at right) in the inoculated leaves of the same plants used in (A). 25S rRNA served as a loading control.