Figure 5.

RDR1-Dependent Production of CMV siRNAs in Arabidopsis.

Detection of CMV genomic/subgenomic RNAs ([A] and [C]) and (−)-strand CMV siRNAs ([B] and [D]) from the inoculated ([C] and [D]) and upper systemically infected leaves ([A] and [B]) of Arabidopsis seedlings of various genotypes as indicated at 5 d ([A] and [B]) and 14 d ([C] and [D]), respectively, after inoculation with CMV (wt) or CMV-Δ2b (Δ). 25S RNA served as a loading control. The CMV RNA species are indicated at right. The membrane was also probed for U6 RNA. Positions of the 21-, 22-, and 24-nucleotide (nt) siRNAs are indicated.